A 8738 4C DRIVER DETAILS:
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A 8738 4C DRIVER
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Correlation between two biological replicates of the apterous A 8738 4c viewpoint for whole embryo tissue at 6—8 h after fertilization. In the plot, the pairwise distribution of count values per fragment is shown. The x - and y -axes drawn in logarithm-like scale, with zero correspond to the counts for the fragments in two biological replicate libraries for the same viewpoint and biological condition. The replicates show good concordance for higher count values. Fragments with 0 counts for both replicates are not shown. The count values usually span several orders of magnitude.
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If a logarithmic transformation were used for the count values, low abundance fragments would tend to show large standard deviations across samples. On the other hand, if untransformed data were used, the standard deviations across samples a 8738 4c be large for high abundance fragments.
Such heteroscedasticity would skew the analysis toward either the fragments far from a 8738 4c close to the viewpoint. Therefore, we use the variance-stabilizing transformation v as introduced by Anders and Huber and implemented in the DESeq2 package Love et al.
After transformation the standard deviations show less dependence on the fragment abundance Fig. Variance-stabilizing transformation. For each fragment, the mean and standard deviation of its count data were computed across all samples for the apterous CRM viewpoint. The plots visualize the distributions of these values for all a 8738 4c. Fragments close to the viewpoint are on the right side with higher count values.
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